If you transform a cell with an alien piece of DNA only, What is the possibility?

1.      This alien piece becomes a part of the host genome.

2.      It doesn't become a part of the host's genome but keeps on replicating

3.      The cloning of genes won't get affected in the absence of Origin of replication.

4.     Multiple identical copies of an alien piece of DNA can be formed irrespective of its integration into the host's genome.

What does cloning mean?

1.      The ability to multiply the desired gene in the host

2.      The ability to multiply the vector in the host

3.      The ability to multiply the antibiotic resistance gene in the host

4.     The ability to multiple Ori in the host

Elution is:

1. Separating the restricted DNA fragments on agarose gel.

2. Staining the separate DNA fragments with ethidium bromide

3. cutting out of the separated band of DNA from the agarose gel and extracting them from the gel piece.

4. constructing rDNA by joining the purified DNA fragments to the cloning vector.

Which of the following step is first one in order to separate DNA fragments?

1.      Running of gel

2.      Staining of gel

3.      Elution

4.     Setting of gel

Which of the following step is last for isolating the desired DNA fragment?

1.      Running of gel

2.      Elution

3.      Staining

4.     Visualization

 E.coli is  a commonly used host for gene cloning because

1. It is free from elements that interfere with replication and recombination of DNA

2. It is easy to transform

3. It supports replication of inserted DNA.

4. All of these

A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:

1. DNA is a hydrophilic molecule

2. DNA is a very large molecule

3. there are no receptors for DNA on the cell membrane

4. DNA is an inert molecule

Two bacteria found to be very useful in genetic engineering are

In order to make the host cell competent

Divalent cations such as calcium are used-

1.      The divalent ions have to be in a specific concentration

2.      They cause the DNA uptake by the cell

3.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

4.     All of the above

When isolating the pure DNA from a bacterial cell, the cell should not be treated with:

1. lysozyme

2. proteases

3. ribonuclease

4. deoxyribonuclease