- 1(current)
Q. No.What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
| I: | At A, same restriction enzyme is used to cut both foreign and vector DNA |
| II: | The enzyme used at B is DNA ligase |
| III: | Step C can be called as transformation |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology non-recombinants transformed bacteria grow on the medium containing ampicillin as well as medium containing tetracycline |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is correct but (R) is not correct. |
| 2. | (A) is not correct but (R) is correct. |
| 3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Given below are the three basic steps in genetically modifying an organism
a. Introduction of the identified DNA into the host.
b. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
c. Identification of DNA with desirable genes.
Choose the option with respect to correct sequence of the given steps
(1) a → c → b
(2) c → b → a
(3) c → a → b
(4) b → a → c
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Histones | 2. | Polysaccharides |
| 3. | RNA | 4. | DNA |
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Following enzymes/techniques are used in the process of recombinant DNA technology
a. EcoRl to cut the isolated genome
b. DNA ligase
c. Protease and ribonuclease for removal of proteins and RNA from DNA
d. Production of recombinant hosts
e. Lysozyme for isolation of the genetic material (DNA)
f. Gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use
(1) c, e, b, f, a, d
(2) e, c, a, b, f, d
(3) e, c, a, f, b, d
(4) a, e, c, b, d, f
- 1(current)
Q. No.
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