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| 1. | binds to troponin to remove the masking of active sites on actin for myosin. |
| 2. | activates the myosin ATPase by binding to it. |
| 3. | detaches the myosin head from the actin filament. |
| 4. | prevents the formation of bonds between the myosin cross bridges and the actin filament. |
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