The experimental proof for semiconservative replication of DNA was first shown in a:
1. Fungus
2. Bacterium
3. Plant
4. Virus
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
Assertion (A): | Semiconservative replication was experimentally proved by Mathew Meselson and Franklin Stahl (1958) |
Reason (R): | Meselson and Stahl used radioactive isotope \(^{15}N\) and equilibrium density gradient centrifugation technique. |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
What would be the proportions of light and hybrid density DNA molecule, respectively if Meselson and Stahl's experiment was continued for 60 minutes?
1. 50%, 50%
2. 25%, 75%
3. 75%, 25%
4. 100%, 0%
1. | The DNA dependent RNA polymerase catalyses polymerization in one direction, that is 5' \(\rightarrow\) 3' |
2. | The DNA dependent DNA polymerase catalyses polymerization in 5' \(\rightarrow\) 3' as well as the 3' \(\rightarrow\) 5' direction. |
3. | The DNA dependent DNA polymerase catalyses polymerization in 5' \(\rightarrow\) 3' direction. |
4. | The DNA dependent DNA polymerase catalyses polymerization in one direction which is 3' \(\rightarrow\) 5'. |
DNA replication is semi-conservative in nature was experimentally proved in eukaryotes by:
1. Hershey and Chase
2. Macleod and McCarty
3. Meselson and Stahl
4. Talyor and his colleagues
Choose the correct pair from the following :
1. | Polymerases | - | Break the DNA into fragments |
2. | Nucleases | - | Separate the two strands of DNA |
3. | Exonucleases | - | Make cuts at specific positions within DNA |
4. | Ligases | - | Join the two DNA molecules |
Taylor conducted the experiments to prove semi-conservative mode of chromosome replication on:
1. Vinca rosea
2. Vicia faba
3. Drosophila melanogaster
4. E. Coli
Statement I: | DNA polymerases catalyse polymerisation only in one direction, that is 5' →3' |
Statement II: | During replication of DNA, on one strand the replication is continuous while on other strand it is discontinuous. |