Q. No.Which of the following is most used in genetic engineering:
1. E. coil and Agrobacterium
2. Mycobacteria and Salmonella
3. Aspergillus
4. Penicillium
Subtopic: Cloning Vector |
93%
From NCERT
AIPMT - 1998
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Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
Subtopic: Cloning Vector |
97%
From NCERT
AIPMT - 2010
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In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
| A. | a bacterium | B. | plasmid |
| C. | plasmodium | D. | bacteriophage |
Options:
| 1. | (A) only | 2. | (A) and (C) only |
| 3. | (B) and (D) only | 4. | (A), (B) and (D) only |
Subtopic: Cloning Vector |
62%
From NCERT
AIPMT - 2010
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
Subtopic: Cloning Vector |
78%
From NCERT
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
72%
From NCERT
NEET - 2024
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
90%
From NCERT
NEET - 2024
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
Subtopic: Cloning Vector |
83%
From NCERT
NEET - 2023
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Match List - I with List - II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
52%
From NCERT
NEET - 2023
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Which of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic: Cloning Vector |
73%
From NCERT
NEET - 2022
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
86%
From NCERT
NEET - 2022
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