Q. No.The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
An enzyme catalysing the removal of nucleotides from ends of DNA is:
1. DNA ligase
2. Endonuclease
3. Exonuclease
4. Protease
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. Eco Rl
2. taq polymerase
3. polymerase lll
4. ligase
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
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