- 1(current)
Q. No.The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
| 1. | Escherichia coli | 2. | Bacillus thuringiensis |
| 3. | Salmonella typhimurium | 4. | Agrobacterium tumefaciens |
Subtopic: Â Process of Biotech |Â Introduction & History |
 74%
From NCERT
NEET - 2022
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Gel electrophoresis is used for:
| 1. | cutting of DNA into fragments |
| 2. | separation of DNA fragments according to their size |
| 3. | construction of recombinant DNA by joining with cloning vectors |
| 4. | isolation of DNA molecule |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 91%
From NCERT
AIPMT - 2008
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While isolating DNA from bacteria, which of the following enzymes is not required?
1. Lysozyme
2. Ribonuclease
3. Deoxyribonuclease
4. Protease
Subtopic: Â Process of Biotech |Â Obtaining Copy of Gene from Donor DNA |Â Host & Desired DNA |
 75%
From NCERT
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
Choose the correct answer from the options given below:
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Â Process of Biotech |
From NCERT
NEET - 2023
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Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 71%
From NCERT
NEET - 2020
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Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 74%
From NCERT
NEET - 2020
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Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
| (a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| (b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
| (c) | The smaller the DNA fragment, the greater the distance it travels through it. |
| (d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
| 3. | (b), (c) and (d) | 4. | (a), (b) and (d) |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
From NCERT
NEET - 2019
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- 1(current)
Q. No.
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