Q. No.Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics
In bacteria, the plasmid is:
1. Extrachromosomal material
2. Main DNA
3. Non-functional DNA
4. Repetitive gene
Manipulation of DNA in genetic engineering became possible due to the discovery of:
1. Restriction endonuclease
2. DNA ligase
3. Transcriptase
4. Primase
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
1. D and E only
2. B and C only
3. A and E only
4. A and B only
1. Restriction enzymes
2. DNA ligase
3. RNA polymerase
4. DNA polymerase
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
| 1. | recognition sequence | 2. | cloning site |
| 3. | restriction site | 4. | ori site |
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