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Q. No.What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. A and E only
4. A and B only
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. A and E only
4. A and B only
Subtopic: Principles of Biotechnology |
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
1. 6 bp
2. 4 bp
3. 10 bp
4. 8 bp
1. 6 bp
2. 4 bp
3. 10 bp
4. 8 bp
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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The " Ti plasmid " of Agrobacterium tumefaciens stands for
1. Tumor independent plasmid
2. Tumor inducing plasmid
3. Temperature independent plasmid
4. Tumour inhibiting plasmid
1. Tumor independent plasmid
2. Tumor inducing plasmid
3. Temperature independent plasmid
4. Tumour inhibiting plasmid
Subtopic: Tools: Vectors: I |
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Which of the following statements is incorrect?
| 1. | Most commonly used bio-reactors are of stirring type. |
| 2. | Bio-reactors are used to produce small scale bacterial cultures. |
| 3. | Bio-reactors have an agitator system, an oxygen delivery system and foam control system. |
| 4. | A bio-reactor provides optimal growth conditions for achieving the desired product. |
Subtopic: Bioreactors/Downstream Processing |
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
Subtopic: Principles of Biotechnology |
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA moves toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Separation and Isolation of DNA fragments |
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
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