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Q. No.The European Federation of Biotechnology [EFB] defines biotechnology as:
| 1. | The use of living cells and bacteria in industrial and scientific processes. |
| 2. | The integration of natural sciences and organisms, cells, parts thereof, and molecular analogues for products and services. |
| 3. | The use of biology to solve problems and make useful products. |
| 4. | The use of biology to develop new products, methods and organisms intended to improve human health and society. |
Subtopic: Introduction & History: I |
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In a broad context, how many of the following could be included in ‘biotechnology’?
| I: | making curd, bread or wine |
| II: | use of genetically modified organisms to produce therapeutic proteins |
| III: | in vitro fertilisation leading to a ‘test-tube’ baby |
| IV: | synthesizing a gene and using it |
| V: | developing a DNA vaccine |
| VI: | correcting a defective gene |
| 1. | 3 | 2. | 4 |
| 3. | 5 | 4. | 6 |
Subtopic: Process of Biotech |
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Two core techniques that enabled birth of modern biotechnology are:
| A: | Techniques to alter the chemistry of genetic material, to introduce these into host organisms and thus change the phenotype of the host organism. |
| B: | Maintenance of sterile ambience in chemical engineering process to enable growth of only the desired microbes/eukaryotic cell in large quantities for the manufacture of biotechnological products. |
| 1. | A is called as Genetic Engineering and B is called as Biophysical engineering. |
| 2. | A is called as Genomics and B is called as Bioprocess engineering. |
| 3. | A is called as Genetic Engineering and B is called as Bioprocess engineering. |
| 4. | A is called as Genomics and B is called as Biophysical engineering. |
Subtopic: Introduction & History |
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Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. |
| 2. | developing methods and software tools for understanding biological data. |
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). |
| 4. | maintenance of sterile ambience in chemical engineering processes. |
Subtopic: Introduction & History: I |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Selectable Markers | Tools |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
Subtopic: Cloning Vector |
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
Subtopic: Cloning Vector |
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
Subtopic: Restriction Enzymes - Main Enzymes |
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The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
| 1. | Salmonella typhimurium | 2. | Escherechia coli |
| 3. | Agrobacterium tumefaciens | 4. | Haemophilus influenzae |
Subtopic: Restriction Enzymes - Main Enzymes | Cloning Vector |
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In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
Subtopic: Cloning Vector |
78%
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