Column I | Column II | ||
a. | Restriction endonuclease | (i) | Hind II |
b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
c. | First restriction endonuclease | (iii) | Isolated in 1963 |
d. | EcoRI | (iv) | Developed in 1972 |
Statement (A): | DNA cannot pass through cell membranes as it is a hydrophobic molecule. |
Statement (B): | Treatment with Ca2+ ions increases the efficiency with which DNA enters into the bacterium. |
Column-I | Column -II | ||
a. | Biosynthetic stage | (i) | Increased surface area for oxygen transfer |
b. | Downstream processing | (ii) | Large-scale production in the fermenter |
c. | Sparged stirred-tank bioreactor |
(iii) | With foam breaker and flat bladed impeller |
d. | Simple stirred-tank bioreactor |
(iv) | Separation and purification |
Statement A: | Wine making as well as antigen production in recombinant organisms are included in biotechnology |
Statement B: | Biotechnology is a very wide area which deals with techniques of using a natural organism (or its parts) as well as genetic engineering |
Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
Column I | Column II | ||
a. | Aniline blue | (i) | Used to stain DNA in agarose gel electrophoresis |
b. | Bromophenol blue | (ii) | stains chromosomes |
c. | Ethidium bromide | (iii) | Tracking dye |
d. | Acetocarmine | (iv) | Used to stain collagen |