Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
1. electroporation
2. liposome transfer
3. microinjection
4. particle bombardment
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. Eco Rl
2. taq polymerase
3. polymerase lll
4. ligase
Match the following enzymes with their functions:
Column-I |
Column-II |
||
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
Options: | (a) | (b) | (c) | (d) |
1. | (iii) | (i) | (iv) | (ii) |
2. | (iii) | (iv) | (i) | (ii) |
3. | (iv) | (iii) | (i) | (ii) |
4. | (ii) | (iv) | (i) | (iii) |
Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides