DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
1. | cutting of DNA into fragments |
2. | separation of DNA fragments according to their size |
3. | construction of recombinant DNA by joining with cloning vectors |
4. | isolation of DNA molecule |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases