- 1(current)
Q. No.Consider the given statements and select the option that correctly states them as true (T) or false (F).
| (a) | A gene whose expression helps to identify transformed cell is known as a selectable marker. |
| (b) | BOD incubator is essentially required for growing microbes on a large scale, for industrial production of enzymes. |
| (c) | For transformation, micro-particles coated with DNA to be bombarbed using gene gun are made up of gold or tungsten. |
| (a) | (b) | (c) | |
| 1. | T | T | T |
| 2. | T | F | T |
| 3. | T | F | F |
| 4. | F | F | T |
Subtopic: Â Selection of Recombinant Transformants |
Level 3: 35%-60%
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| Assertion: | E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline. |
| Reason: | Recognition site for Bam HI is present in tetR region of pBR322. |
| 1. | Both assertion and reason are true, and the reason is the correct explanation of the assertion. |
| 2. | Both assertion and reason are true, but the reason is not the correct explanation of the assertion. |
| 3. | Assertion is true but reason is false. |
| 4. | Both assertion and reason are false. |
Subtopic: Â Selection of Recombinant Transformants |
Level 3: 35%-60%
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Assertion(A): Only antibiotic resistant gene in vectors can be used as selectable markers.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Selection of Recombinant Transformants |
Level 3: 35%-60%
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- 1(current)
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