The Taq polymerase enzyme is obtained from
1. Thiobacillus ferroxidans
2. Bacillus subtilis
3. Pseudomonas subtilis
4. Thermus aquaticus
The thermostable enzyme Taq polymerase used in PCR technique was discovered by
(1)Alec Jeffrey
(2)Karry Mullis
(3)Temin
(4)Baltimore
What is true about the steps of PCR shown in the diagram given below?
| I: | Step A occurs at the temperature of 95 – 980 C |
| II: | Step B occurs at the temperature of 550 C |
| III: | Step C occurs at the temperature of 720 C |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension
Which one is a true statement regarding DNA polymerase used in PCR?
| 1. | It is used to ligate introduces DNA in recipient cells |
| 2. | It serves as a selectable marker |
| 3. | It is isolated from a virus |
| 4. | It remains active at high temperatures |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents.
| 1. | A - Denaturation at a temperature of about 50ºC |
| 2. | C - Extension in the presence of heat-stable DNA polymerase |
| 3. | A - Annealing with two sets of primers |
| 4. | B - Denaturation at a temperature of about 98ºC separating the two DNA strands |
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
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