Biotechnology and its applications - Live Session Contact Number: 9667591930 / 8527521718Biotechnology and its applications - Live Session Contact Number: 9667591930 / 8527521718Select the incorrect statement with respect to Green-Revolution.
1. Succeeded in quadrupling the food supply
2. Increase was insufficient to feed the growing human population
3. Increased yields could be attributed to improved crop varieties
4. Use of agrochemicals contributed to enhanced yields
Which of the following is not an advantage of GM crops?
1. Increased crop tolerance to salt in soil
2. Increased reliance on chemical pesticides
3. Reduction in post harvest losses
4. Increased efficiency of mineral usage
Identify the gene and its protein that controls corn borer from the given options
1. cry l Ab and Cry l Ab
2. cry l Ac and Cry l Ac
3. cry ll Ab and Cry ll Ab
4. cry l Ac and Cry l Ac
Crystalline protein synthesized by Bacillus thuringiensis is activated by
1. Acidic conditions of bacterial foodsvacuole
2. Alkaline pH of bacterial food vacuole
3. Acidic pH in insect fore-gut
4. Alkaline pH insect, mid-gut
How many recombinant therapeutics have been approved for human use in world over?
1. 12
2. 30
3. 42
4. 27
Removal of which chain results in maturation of proinsulin to insulin?
1. D chain
2. C chain
3. B chain
4. Achain
Which enzyme was targetted during the first clinical gene therapy given in 1990 to a four year- old girl?
1. Monoamine oxidase
2. Tyrosine oxidase
3. Pyruvate dehydrogenase
4. Adenosine deaminase
Animals that have had their DNA manipulated to possess and express an extra gene are known as
1. Foreign animals
2. Superior animals
3. Transgenic animals
4. Elevated animals
About what percentage of all existing transgenic animals are mice?
1. 75
2. 95
3. 100
4. 50
If a probe is made of radioactively labelled dsDNA and is allowed to hybridise to its complementary DNA in a cloned cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?
1. It is double stranded DNA
2. It is radioactively labelled
3. This probe can be used in autoradiography
4. It is hybridise to complementary DNA/RNA
Techniques that serve the purpose of early diagnosis include
1. PCR
2. ELISA
3. Serum analysis
4. Both 1 & 2
Which of the following protein product has been used to treat emphysema?
1. -lactalbumin
2. TPA
3. -1-antitrypsin
4. C-peptide
'Rosie' a transgenic cow is known to produce a type of milk which has all the following charcteristics, except
1. Protein content of 2.4 g/L
2. Human -lactalbumin
3. More balanced diet than normal cow milk for babies
4. Was produced for first time in year 2001
The bacterium Bacillus thuringiensis is widely used in contemporary biology as
1. Bioweapon
2. Bioinsecticide
3. Bioweedicide
4. Indicator of water pollution
Which of the following is a transgenic plant?
1. Flavr savr
2. Meloidogyne incognita
3. Caenorhabditis elegans
4. Oryza sativa
Select incorrect statement with respect to RNAi
1. dsDNA binds target mRNA and initiate RNAi
2. Agrobacterium vector is used to introduce nematode specific gene into host plant
3. ssRNA binds target mRNA and initiate RNAi
4. Both 2 & 3
Infestation by tobacco budworm and armyworm is prevented by Bt toxin. These insects belong to order
1. Coleoptera
2. Lepidoptera
3. Diptera
4. Hymenoptera
Which of the following is correct statement with respect to animal insulin?
1. It is just as effective as human insulin over prolonged time
2. It does not elicit immune response ever
3. It can be orally administrated to diabetic people
4. It was extracted from pancreas of slaughtered cattle and pigs
Technique used to detect HIV in suspected AIDS patient in asymptomatic stage is
1. PCR
2. Serum analysis
3. Both 1 & 2
4. Urine analysis
The use of bioresources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is called
1. Bioethics
2. Biopiracy
3. Bioterror
4. Bioweapon
Which ingredient was present in higher concentrations in GM rice as compared to the usual rice?
1. Protein
2. Carbohydrate (starch)
3. -carotene
4.
Select the incorrect match
1. ery l Ab - Com borer
2. Bt toxin - Beetles
3. cry l Ac - Cotton Bollworm
4. cry ll Ac - Cotton Bollworm
Enzyme used in ELISA test is
1. Endonuclease
2. Ligase
3. Peroxidase
4. Polymerase
A probe is used in which stage of genetic engineering?
1. Cleaving DNA
2. Recombining DNA
3. Cloning
4. Screening
What could be the possible harm of using antibiotic resistant gene as a selectable marker in plasmid, used for gene transfer in production of GM food?
(A) GM food contains the enzyme produced by the antibiotic resistance gene that was used during gene tranfer in genetic engineering. This could cause allergies since it is a foreign protein.
(B) The bacteria present in the alimentary canal of the humans could take up the antibiotic resistance gene present in GM food.
(C) The bacteria in the human alimentary canal would then become resistant to the concerned antibiotic.
(D) The transgene may be transferred through pollen to their wild relatives and make weeds more persistant and damaging.
1. (A) only
2. Both (B) & (C)
3. (A), (B) & (C)
4. (A), (B), (C) & (D)
The introduction of t-DNA into plant involves
1. Allowing the plant roots to stand in water
2. Infection of the plant by Agrobacterium tumefaciens
3. Altering the pH of the soil, then heat-shocking the plants
4. Exposing the plants to cold for a brief period
Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?
1. Research Committee on Genetic Manipulation
2. Bio-safety committee
3. Indian Council of Agricultural Research
4. Genetic Engineering Approval Committee
Commonly used vectors for human genome sequencing are
1. T-DNA
2. BAC and YAC
3. Expression vectors
4. T/A cloning vectors
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
1. Both sense and anti-sense RNA
2. A particular hormone
3. An antifeedant
4. A toxic protein
Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of R-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which of the two statements having mistakes?
1. (A) and (C)
2. (A) and (B)
3. (B) and (C)
4. (C) and (D)
Polyethylene glycol method is used for
1. Biodiesel production
2. Seedless fruit production
3. Energy production from sewage
4. Gene transfer without a vector