A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :
1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin
A gene, whose expression helps to identify transformed cells is known as
1. selectable marker
2. vector
3. plasmid
4. structural gene
How are transformants selected from nontransformants?
1. | Presence of more than one recognition site in the vector DNA. |
2. | Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker. |
3. | Antibiotic resistance gene gets inactivated due to insertion of alien DNA. |
4. | Both 2 and 3. |
Why is a marker gene useful?
1. It shows you whether the gene being added, has been taken up or not.
2. It's a way of labelling which gene you want to modify.
3. It sticks with the striker gene.
4. It marks the position of r-DNA
Selectable marker helps
1. To identify transformants
2. To identify recombinants
3. To identify non- recombinants
4. To eliminate transformants