In the screening process during rDNA experiments, clones that metabolize -gal turn:
1. Colorless
2. Blue
3. Yellow
4. Green
How are transformants selected from nontransformants?
1. | Presence of more than one recognition site in the vector DNA. |
2. | Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker. |
3. | Antibiotic resistance gene gets inactivated due to insertion of alien DNA. |
4. | Both 2 and 3. |
Insertional inactivation of a gene helps in
1. | Identification of recombinant clones |
2. | Identification of deletion mutants |
3. | Identification of recombinant transformants |
4. | Elimination of suppression mutants |
Insertional inactivation of the lac Z gene forms-
1. | Blue recombinant colonies |
2. | Colourless recombinant colonies |
3. | Fluorescent green colonies |
4. | There is no relation between the lac Z gene and colour of the colony. |
Which of the following would you choose as the safest and least cumbersome selectable marker-
1. | Ampicillin resistance gene |
2. | Tetracyclin resistance gene |
3. | Kanamycin resistance gene |
4. | b Galactosidase gene |