Fertilisation in humans is practically feasible only if

1. the ovum and sperms are transported simultaneously to ampullary-isthmic junction of the fallopian tube
2. the ovum and sperms are transported simultaneously to ampullary-isthmic junction of the cervix
3. the sperms are transported into cervix within 48 hrs of release of ovum in uterus
4. the sperms are transported into vagina just after the release of ovum in fallopian tube

In human females, meiosis-II is not completed until 

1. Puberty

2. fertilization 

3. Uterine implantation 

4. birth 

Assertion   :       Penetration of sperm into ovum is a chemical process.

Reason      :       Acrosome of sperm secretes a lytic enzyme hyaluronidase which dissolves vitelline membrane of ovum.

Assertion   :       Vagina acts as copulation canal and fertilization canal.

Reason      :       Both insemination and fusion of gametes occur in vagina of female.

What is the order of layers or space from outside to inside?