The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI

2. HindII

3. SmaI

4. BamHI

The following palindrome is recognized by the restriction enzyme:

1. BamHI

2. EcoRI

3. HindII

4. PstI

Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid

2. Bacteriophages

3. BAC

4. Plasmid

The DNA fragments separated on an agarose gel can be visualised after staining with
1. Bromophenol blue
2. Acetocarmine
3. Aniline blue
4. Ethidium bromide

Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
    3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
    3' - GAATCC - 5'
3. 5' - CATTAG - 3'
    3' - GATAAC - 5'
4. 5' - GATACC - 3'
    3' - CCTAAG - 5'

Restriction endonucleases present in bacterial cells provide an advantage to the cell because these enzymes:

Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?

DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol 
3. Chilled ethanol
4. Methanol at room temperature

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:

Biolistic technique is used in:

1. Gene transfer process

2. Tissue culture process

3. Hybridization process

4. Germplasm conservation process