The technique of DNA fingerprinting was developed initially by:
1. | Alfred Sturtevant | 2. | Alec Jeffrys |
3. | Thomas Cech | 4. | Fire and Mello |
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Southern blotting is transfer to nitrocellulose sheet from agar gel of:
1. | RNA | 2. | Proteins |
3. | DNA | 4. | Lipids |
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The size of VNTR varies from:
1. 0.1 to 20 kb
2. 0.1 to 2.0 mb
3. 0.1 to 0.2 bp
4. 0.1 to 2.0 bp
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Griffith co-injected the heat-killed S and live R strains of Pneumococcus bacterium into the mice and much to his surprise the mice developed pneumonia and died. He concluded that:
I: | Live R was transformed into Live S strain as he was able to isolate Live S strain from the blood of the infected mice. |
II: | Bacterial transformation is a stable and heritable change as the culture of bacteria isolated from dead mice was unable to infect other mice. |
1. | Both I and II are correct |
2. | Only I is correct |
3. | Only II is correct |
4. | Both I and II are incorrect |
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The radius of the double helix of B-DNA [Watson and Crick model] is approximately:
1. | 1.0 nm | 2. | 2.0 nm |
3. | 0.34 nm | 4. | 3.4 nm |
An RNA primer is essential for DNA synthesis carried out by DNA polymerase III. This is because, to synthesize DNA, DNA polymerase III requires:
1. | A free 3’ – PO4 group |
2. | A free 5’ – PO4 group |
3. | A free 3’ – OH group |
4. | A free 5’ – OH group |
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Which of the following possible modes of replication was eliminated by Meselson and Stahl based on the finding after one generation of replication of the bacterium?
1. | Dispersive | 2. | Semi-conservative |
3. | Conservative | 4. | Both 1 and 3 |
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The mRNA codon GUG codes for:
1. | Valine | 2. | Glutamic acid |
3. | Tryptophan | 4. | Formylated methionine |
What defines the template and coding strand of DNA for transcription?
1. Presence of the structural gene in a transcription unit
2. The core RNA polymerase enzyme
3. Presence of the promoter in a transcription unit
4. Presence of 3’ – 5’ TAC codon on the DNA template