The technique of DNA fingerprinting was developed initially by:

1.Alfred Sturtevant2.Alec Jeffrys
3.Thomas Cech4.Fire and Mello

Subtopic:  DNA Fingerprinting |
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Southern blotting is transfer to nitrocellulose sheet from agar gel of:

1.RNA2.Proteins
3.DNA4.Lipids

Subtopic:  DNA Fingerprinting |
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The size of VNTR varies from:

1. 0.1 to 20 kb
2. 0.1 to 2.0 mb
3. 0.1 to 0.2 bp
4. 0.1 to 2.0 bp

Subtopic:  Human Genome Project | DNA Fingerprinting |
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For the multiplication of any alien piece of DNA in an organism it needs to be a part of a chromosome that has a specific:
1. Telomeric sequence
2. Multiple cloning site
3. Ori
4. Selectable marker

Subtopic:  DNA Replication |
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Griffith co-injected the heat-killed S and live R strains of Pneumococcus bacterium into the mice and much to his surprise the mice developed pneumonia and died. He concluded that:

I: Live R was transformed into Live S strain as he was able to isolate Live S strain from the blood of the infected mice.
II: Bacterial transformation is a stable and heritable change as the culture of bacteria isolated from dead mice was unable to infect other mice.
 
1. Both I and II are correct
2. Only I is correct
3. Only II is correct
4. Both I and II are incorrect

Subtopic:  Search for Genetic Material |
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The radius of the double helix of B-DNA [Watson and Crick model] is approximately:

1.1.0 nm2.2.0 nm
3.0.34 nm4.3.4 nm

Subtopic:  DNA Double Helix |
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An RNA primer is essential for DNA synthesis carried out by DNA polymerase III. This is because, to synthesize DNA, DNA polymerase III requires:

1.  A free 3’ – PO4 group
2.  A free 5’ – PO4 group
3.  A free 3’ – OH group
4.  A free 5’ – OH group

Subtopic:  DNA Replication |
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Which of the following possible modes of replication was eliminated by Meselson and Stahl based on the finding after one generation of replication of the bacterium?

1. Dispersive 2. Semi-conservative
3. Conservative 4. Both 1 and 3

Subtopic:  Search for Genetic Material |
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The mRNA codon GUG codes for:

1. Valine 2. Glutamic acid
3. Tryptophan 4. Formylated methionine
Subtopic:  Translation |
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What defines the template and coding strand of DNA for transcription?
1. Presence of the structural gene in a transcription unit

2. The core RNA polymerase enzyme

3. Presence of the promoter in a transcription unit

4. Presence of 3’ – 5’ TAC codon on the DNA template

Subtopic:  Transcription: I |
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