Q. No.The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:
1.
Salmonella typhimurium
2.
Escherechia coli
3.
Agrobacterium tumefaciens
4.
Haemophilus influenzae
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The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:
| 1. | depends on a particular or sequence of the main chromosomal DNA |
| 2. | is possible due to promoter sequence present in the insert |
| 3. | cannot occur without telomeric sequences |
| 4. | is independent of the control of chromosomal DNA |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
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In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
| 1. | 1 and 2 | 2. | 3 and 4 |
| 3. | 6 and 7 | 4. | 5 and 8 |
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A piece of DNA is inserted within the gene of beta-galactosidase in the E.coli plasmid. X-gal is added to the medium during screening. The colonies of recombinant transformants:
| 1. | will show an increase in growth |
| 2. | will not be able to survive |
| 3. | will give a deep blue color |
| 4. | will not produce any color |
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In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:
| 1. | will move towards anode with smaller fragments moving farther than the larger segments |
| 2. | will move towards anode with larger fragments moving farther than the smaller segments |
| 3. | will move towards cathode with smaller fragments moving farther than the larger segments |
| 4. | will move towards cathode with larger fragments moving farther than the smaller segments |
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If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA polymerase | 4. | Chitinase |
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
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