Q. No.Identify the incorrect statement regarding structure of DNA:
1. Pitch is 3.4nm and 10 base pairs.
2. The two strands are antiparallel.
3. Each pair of nucleotides is held together by three hydrogen bonds.
4. The diameter of the molecule is a uniform 2nm.
1. Pitch is 3.4nm and 10 base pairs.
2. The two strands are antiparallel.
3. Each pair of nucleotides is held together by three hydrogen bonds.
4. The diameter of the molecule is a uniform 2nm.
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T2 bacteriophage was used by Hershey and Chase to conclusively prove that DNA and not protein is the genetic material because:
| 1. | It makes capsomeres with the help of its genes. |
| 2. | It injects its genetic material into a bacterial cell. |
| 3. | It can undergo either the lytic or lysogenic life cycle |
| 4. | It enters the bacterial cell to cause infection. |
Chromatin is:
| 1. | Complete genetic sequences contained by members of a particular species. |
| 2. | The DNA-protein complex comprising of eukaryotic chromosomes. |
| 3. | Tandem repetitive sequences in the non-coding DNA. |
| 4. | All the transcription units in the genome. |
The nitrogenous bases purines and pyrimidines basically differ in that:
| 1. | The former is found in RNA while the latter is found in DNA. |
| 2. | The latter is found in RNA while the former is found in DNA. |
| 3. | The former contains a double-ring backbone, while the latter contains a single-ring backbone. |
| 4. | The latter contains a double-ring backbone while the former contains a single-ring backbone. |
Identify the correct statements regarding a replisome:
| I: | It is a complex of several proteins. |
| II: | It includes enzymes like DNA polymerase, DNA helicase, and primase. |
| III: | It is found at the replication fork during DNA synthesis. |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
The basic difference between the two key enzymes, DNA pol III and DNA ligase, required for DNA replication is:
| 1. | Only DNA pol III synthesizes phosphoester bonds. |
| 2. | Only DNA ligase synthesizes phosphoester bonds. |
| 3. | DNA pol III can synthesize DNA from 3'-5'. |
| 4. | DNA ligase can use energy from ATP rather than nucleotides. |
In which models of DNA replication In this model the two parental DNA strands are back together after replication has occurred?
1. Conservative model.
2. Semiconservative model.
3. Dispersive model.
4. Destructive model.
If a bacterial cell were to contain 4 nucleoids per cell, the number of copies of the bacterial chromosome present in each cell will most likely be:
1. 1
2. 2
3. 3
4. 4
Lagging strand is replicated in a discontinuous manner in small Okazaki fragments because:
| 1. | DNA polymerase cannot synthesize large stretches of DNA. |
| 2. | DNA polymerase needs to be replaced every 1000-2000 nucleotide. |
| 3. | DNA polymerase requires a free 3' OH group to attach new nucleotides. |
| 4. | Only one DNA strand can be used as the template at a time |
The lagging strand and the leading strand during DNA replication are replicated differently because:
1. On the leading strand, DNA synthesis occurs 5' to 3' on the leading strand, while on the lagging strand it is 3' to 5'.
2. DNA polymerase is able to continuously add new nucleotides on the leading strand while it must keep 'starting over' on the lagging strand.
3. The lagging strand requires only a single primer while the leading strand requires many.
4. Helicase opens the leading strand at a faster rate than the lagging strand.
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