Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?

1. BamHI

2. Psti

3. Clal

4. Pvull

In the screening process during rDNA experiments, clones that metabolize $\beta$-gal turn:

1. Colorless

2. Blue

3. Yellow

4. Green

Microinjection is suitable for :

1. Injecting an ovum into the sperm in IVF

2. Transforming animal cells

3. Injecting very small sized drug particles into neurons

4. Conferring antibiotic resistance to certain strain of bacteria 

Polymerase Chain Reaction is not used in :

1. Confirming presence of a pathogen during early infection

2. Identifying the mutated genes in suspected cancer patients

3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures

4. Detection of the presence of HIV in suspected AIDS patient

The plasmid pBR322 does not contain:

1. An origin of replication site

2. A gene that encodes for restrictor of plasmid copy number

3. Gene for Ampicillin and Streptomycin resistance

4. Sites for many restriction enzymes

Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

The bioreactor shown in the diagram is:

1. Sparged stirred tank type

2. Airlift type

3. Simple stirred tank type

4. Fluidized bed type

What is true about the steps of PCR shown in the diagram given below?

l. Step A occurs at the temperature of 95-98${}^{\mathrm{o}}$ C

ll. Step B occurs at the temperature of 55${}^{\mathrm{o}}$ C

lll. Step C occurs at the temperature of 72${}^{\mathrm{o}}$ C

1. l and ll only

2. l and lll only

3. ll and lll only

4. l, ll and lll

A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of :

1. BamH I and Sma I

2. Hind II and Sma I

3. BamH I and SaI I

4. SaI I and Hind II

What is true A,B and C in the given diagrammatic representation of rDNA technology ?

I. At A same restriction enzyme is used to cut both foreign and vector DNA

II. The enzyme used at B is DNA ligase

III. Step C can be called as transformation

1. I and II only

2. I and III only

3. II and III only

4. I,II and III