What is true about the steps of PCR shown in the diagram given below?

l. Step A occurs at the temperature of 95-98${}^{\mathrm{o}}$ C

ll. Step B occurs at the temperature of 55${}^{\mathrm{o}}$ C

lll. Step C occurs at the temperature of 72${}^{\mathrm{o}}$ C

1. l and ll only

2. l and lll only

3. ll and lll only

4. l, ll and lll

A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of :

1. BamH I and Sma I

2. Hind II and Sma I

3. BamH I and SaI I

4. SaI I and Hind II

What is true A,B and C in the given diagrammatic representation of rDNA technology ?

I. At A same restriction enzyme is used to cut both foreign and vector DNA

II. The enzyme used at B is DNA ligase

III. Step C can be called as transformation

1. I and II only

2. I and III only

3. II and III only

4. I,II and III

According to traditional view which of the following can be kept under the process or form in Biotechnology?
A. Test tube baby
B. DNA vaccine
C. Making of wine
D. Correcting a gene

If you transfom a cell with an alien piece of DNA only, What is the possibility?

1. This alien piece becomes a part of host genome.

2. It doesn't become a part of host's genome but keeps on replicating

3. The cloning of gene won't get affected in the absence of Origin of replication.

4. Multiple identical copies of alien piece of DNA can be formed irrespective of its integration into host's genome.

In the year 1963, the two enzymes responsible for inhibiting the growth of bacteriophage in E. coli were isolated. One of them was
1.      Ligase
2.      Polymerase
3.      Molecular scissor
4.     Antibiotic resistance enzyme

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1. 700; 350

2. 900; 230

3. 600; 270

4. 550; 210

Restriction endonuclease

A.      Directly recognizes specific sequence

B.      Makes the cut at sugar-phosphate core.

C.      Comes under nuclease

D.     Both A and C

Which of the following step is first one in order to separate DNA fragments?

A.      Running of gel

B.      Staining of gel

C.      Elution

D.     Setting of gel

In a DNA gel the fragment of 2kb and 3kb will be

1. 2kb nearer to cathode while 3kb nearer to anode

2. 2kb closer to wells while 3kb towards the opposite ends

3. 2kb closer to opposite end of gel while 3kb closer to well

4. 2kb away from cathode while 3kb towards anode