If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be
A. Cellulase
B. Protease
C. Ribonuclease
D. Chitinase
If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?
A. Cellulase
B. Chitinase
C. Lysozyme
D. Protease
If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?
A. DNAse
B. Ribonuclease
C. Protease
D. Lysozyme
The precipitation of DNA out of the solution is done by adding
A. Organic Acid
B. HCl
C. Ethanol
D. Ketone
The end result of precipitation of DNA in suspension is seen
A. In the form of dark solution
B. In the form of effervescence
C. In the form of fine threads
D. In the form of bubbles
The precipitated DNA can be removed from the suspension
A. By Filtration
B. By giving a heat shock
C. By spooling
D. By gel electrophoresis
The synthesis of DNA in PCR will be in
1. 5’-3’
2. 3’-5’
3. It won't be depending on direction
4. For one strand 5’-3’ in another opposite to it
The primers designed according to (Answer according to very basic PCR)
1. Terminal ends of vector
2. Terminal ends of gene of interest
3. Random primers
4. According to direction of PCR
Which of the following doesn't go with PCR?
A. Thermus aquaticus
B. 1 billion copies
C. Taq polymerase
D. Dideoxynucleotides
What is the final step of PCR?
A. Extension
B. Annealing
C. Denaturation
D. Extraction