Which of the following statement is not true for origin of replication

1. It’s a definite place where replication originates

2. It should be present on vector DNA for making recombinant DNA

3. It should be present in host in which vector DNA has to be transferred

4. Both b and c

Which principle govern the principle of transcription?

1. Cleavage of phosphodiester bond

2. Phosphorylation

3. Complementarity

4. duplication

Which of the following statement is not true ?

1. In replication the total DNA of an organism gets duplicated

2. In transcription complete one strand of the DNA gets copied

3. In transcription only a segment of only one strands of the DNA gets copied

4. Both b and c

Why both the strands of DNA are not copied during transcription because

1. RNA molecules with different sequences will be produced and hence proteins with different sequences will be produced.

2. double stranded RNA molecules will be produced that would prevent translation of dsRNA

3. cell will fail to produce proteins as no single stranded RNA is present

4. All of the above

Which of the following is not a unit of transcription unit

1. A promoter

2. The structural gene

3. A terminator

4. A operator

The direction of transcription is

1. always from 5’ to 3’

2. always from 3’ to 5’

3. depends on the template direction

4. sometimes 5’ to 3’ and sometimes 3’ to 5’

The strand of DNA which does not code for anything is referred to as

1. anti-sense strand

2. coding strand

3. non-coding strand

4. template strand

which of the following is true for coding strand

1. it’s known as template strand

2. it’s polarity is 5’ to 3’

3. it’s a sense strand

4. Both b and c

Which of the following is true for promoter and terminator of a transcription unit?

1. Both flank the structural gene

2. promoter is upstream of the structural gene with respect to coding strand polarity

3. terminator is downstream of the structural gene with respect to coding strand polarity

4. all of the above

Template and coding strands are defined by the presence of

1. terminator

2. structural gene

3. promoter

4. all of these