Arrange the following steps of a plant breeding program in a correct chronological manner:

I. Selection and testing of superior recombinants

II. Collection of variability

III. Crosshybridization among the selected parents

IV. Evaluation and selection of parents

V. Testing, release and commercialization of new cultivars

1. II, IV, I, III, V

2. IV, I, II, III, V

3. II. IV, III, I, V

4. IV, III, II, I, V

Identify the incorrect statement regarding Multiple Ovulation Embryo Transfer Technology (MOET):

(1) FSH like hormone or PMSG [pregnant mare serum gonadotropin] are used to induce super-ovulation [6-8 eggs rather thanone per cycle] and Prostagladins are used to regulate the duration of cycle.

(2) Fresh embryos can be stored at 37$°$C for 6-8 hours without much harm but it is recommended that the embryos are inserted into the recipient cow or frozen as soon as possible. The embryos can be stored for ever in liquid nitrogen.

(3) The animal is either mated with an elite bull or artificially iseminated and the fertilized eggs at 8-32 cells stages, are recovered non-surgically and transfered to genetic mothers.

(4) The embryos are transferred to a recipient cow whose oestrous cycle is at the correct receptive stage.

The best breeding method for animals that are below average in produtivity in milk production would be:

(1) Inbreeding

(2) Out-crossing

(3) Cross-breeding

(4) Interspecific hybridisation

Identify the option where all columns are not correctly matched:

In mung bean the resistance to yellow mosaic virus and powdery mildew were introduced by:

1. Mutations

2. Hybridization

3. Tissue culture

4. Genetic engineering

Pusa Shubhra, an improved variety of cauliflower, is resistant to:

1. White rust

2. Bacterial blight

3. Black rot

4. Leaf curl

To obtain a virus free plant, what can be used as an explant in micropropogation ?

(1) Apical meristem

(2) Axillary meristem

(3) Either apical or axillary meristem

(4) Embryo

Inbreeding depression in animals can be overcome by:

1. A single outcross

2. Out cross for many generations

3. Cross-breeding

4. Inter-specific hybridization

Identify the incorrectly matched pair:

Meristem culture is especially important to obtain: 

(1) Drought resistant plants

(2) Virus free plants

(3) Biofortified plants

(4) Androgenic haploids