The technique not used for transformation of plant cells in recombinant procedures is:
1. Biolistics
2. Agrobacterium mediation
3. Use of viruses
4. Micro-injection
In the screening process during rDNA experiments, clones that metabolize -gal turn:
1. Colorless
2. Blue
3. Yellow
4. Green
Microinjection is suitable for :
1. Injecting an ovum into the sperm in IVF
2. Transforming animal cells
3. Injecting very small sized drug particles into neurons
4. Conferring antibiotic resistance to a certain strain of bacteria
The plasmid pBR322 does not contain:
1. | An origin of replication site |
2. | A gene that encodes for restrictor of plasmid copy number |
3. | Gene for Ampicillin and Streptomycin resistance |
4. | Sites for many restriction enzymes |
Restriction enzymes are synthesized by:
1. Bacteria only
2. Yeast and bacteria only
3. Eukaryotic cells only
4. All kinds of cells
The bioreactor shown in the diagram is:
1. Sparged stirred tank type
2. Airlift type
3. Simple stirred tank type
4. Fluidized bed type
What is true about the steps of PCR shown in the diagram given below?
I: | Step A occurs at the temperature of 95-98° C |
II: | Step B occurs at the temperature of 55° C |
III: | Step C occurs at the temperature of 72° C |
1. l and ll only
2. l and lll only
3. ll and lll only
4. l, ll and lll
A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of:
1. BamH I and Sma I
2. Hind II and Sma I
3. BamH I and SaI I
4. SaI I and Hind II
What is true A,B and C in the given diagrammatic representation of rDNA technology ?
I: | At A same restriction enzyme is used to cut both foreign and vector DNA |
II: | The enzyme used at B is DNA ligase |
III: | Step C can be called as transformation |
1. | I and II only |
2. | I and III only |
3. | II and III only |
4. | I,II and III |
The process of separation and purification of expressed protein before marketing is called
(1) upstream processing
(2) downstream processing
(3) bioprocessing
(4) postproduction processing