DNA fragments created by the action of restriction enzymes can be separated by agar gel electrophoresis where:
I:
larger the fragment size, the farther it moves in the gel
II:
the DNA fragments are forced to move towards anode under an electric field
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Separation and Isolation of DNA fragments |
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A ‘continuous culture system’ method usually produces a larger biomass mainly because:
| 1. | the environmental conditions for the growth of the microbes are very easily maintained in such a system |
| 2. | such a system keeps most of the cells in their active/log exponential phase |
| 3. | it is very cheap with respect to other systems and hence cost of production is low |
| 4. | such a system uses larger sized bioreactors than other systems |
Subtopic: Large Scale Production |
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Animal cells cannot be transformed with the help of:
| 1. | Microinjection | 2. | Ti Plasmid |
| 3. | Retroviral vectors | 4. | Liposomes |
Subtopic: Transforming Plant & Animal Cell |
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Work of which of the following scientists was the basis upon which the discipline of biotechnology was founded?
| 1. | Cohen and Boyer |
| 2. | Watson and Crick |
| 3. | Hershey and Chase |
| 4. | Avery, Macleod and McCarty |
Subtopic: Restriction Enzymes - Main Enzymes |
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The DNA sequence shown below is:
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
| 1. | the consensus sequence for prokaryotic promoter |
| 2. | the most common VNTR in the human genome |
| 3. | the recognition sequence of a common restriction endonuclease EcoR I |
| 4. | an intron within the eukaryotic split gene |
Subtopic: Restriction Enzymes - Main Enzymes |
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Which of the following statements regarding restriction endonucleases used in recombinant DNA procedures is not true?
| 1. | They are isolated from bacteria |
| 2. | They are synthesized by bacteria as a mechanism of defence against bacteriophages |
| 3. | The type II enzymes, commonly used in rDNA experiments, cut dsDNA at specific recognition sequences |
| 4. | The bacterial DNA does not have recognition sequences for the restriction enzymes produced by itself |
Subtopic: Restriction Enzymes - Main Enzymes |
74%
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It is desirable to use restriction enzymes that create sticky ends in recombinant DNA technology procedures because this:
| 1. | facilitates the action of DNA ligase |
| 2. | makes selection of recombinant DNA possible |
| 3. | increases the copy number of rDNA per cell |
| 4. | will create multiple fragments of the vector DNA |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II | Tools |
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Plasmids are good cloning vectors mainly because:
| 1. | they are circular |
| 2. | they replicate autonomously |
| 3. | they are extra-chromosomal DNA molecules found in many bacterial cells |
| 4. | they carry genes vital for normal survival and reproduction |
Subtopic: Tools: Vectors: I |
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In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Cloning Vector |
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A plasmid has genes for resistance against ampicillin and tetracycline. The tetracycline resistant gene is insertionally inactivated by inserting a foreign DNA within the gene. The bacterial cells are transformed. The recombinant transformants:
| 1. | will be killed in a medium containing ampicillin but not in a medium containing tetracycline |
| 2. | will be killed in a medium containing tetracycline but not in a medium containing ampicillin |
| 3. | will be killed in a medium containing ampicillin and in a medium containing tetracycline |
| 4. | will not be killed in a medium containing ampicillin and in a medium containing tetracycline |
Subtopic: Cloning Vector |
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