Assertion(A):
Insertional inactivation method helps to differentiate recombinant from non-recombinant
Reason(R):
Selection of recombinant in this method depends upon inactivation of antibiotics
1.
Both (A) and (R) are True and (R) is the correct explanation of (A).
2.
Both (A) and (R) are True but (R) is not the correct explanation of (A).
3.
(A) is True but (R) is False.
4.
Both (A) and (R) are False.
Subtopic: Â Selection of Recombinant Transformants |
From NCERT
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Which of the following is true about Stanley cohen and Herbet Boyer experiment ?
| 1. | They used plasmid of salmonella typhimurium as a vector |
| 2. | They used plasmid of Escherichia coli to obtained antibiotic resistance gene |
| 3. | They used molecular scissor’s to obtained antibiotic resistance gene from the plasmid of salmonella typhimurium |
| 4. | They used DNA ligase to join antibiotic resistance gene obtained from Escherichia coli to the plasmid of salmonella |
Subtopic: Â Restriction Enzymes - Main Enzymes |
From NCERT
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Gene transfer involves some essential stages such as
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
| (i) | inserting recombinant DNA into suitable recipient organism |
| (ii) | isolation of a fragment of DNA |
| (iii) | screening of transformed cells |
| (iv) | integrating DNA fragment into suitable vector |
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
Subtopic: Â Transforming Plant & Animal Cell |
 84%
From NCERT
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Â Tools |Â General Design of an rDNA experiment |
 74%
From NCERT
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Which of the following DNA is used by Agrobacterium tumefaciens to transform normal cells into a tumor?
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
Subtopic: Â Transforming Plant & Animal Cell |
 89%
From NCERT
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Selection of recombinations based on their inability to produce colour in the presence of a chromogenic substrate is
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
Subtopic: Â Cloning Vector |
 89%
From NCERT
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Restriction endonucleases are bacterial enzymes that cut double stranded DNA. These restriction enzymes do not cut their own DNA because
| 1. | The bacterium does not contain the specific recognition sequence |
| 2. | The DNA is packed with histones |
| 3. | The specific recognition sequences are masked with methyl groups |
| 4. | Restriction endonucleases cut viral DNA only |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 82%
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Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: Â General Design of an rDNA experiment |
 79%
From NCERT
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NEET 2025 - Target Batch
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NEET 2025 - Target Batch
Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R).
In light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True and (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is True. |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 61%
From NCERT
NEET - 2022
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NEET 2025 - Target Batch
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Â Separation and Isolation of DNA fragments |
 91%
From NCERT
NEET - 2022
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NEET 2025 - Target Batch
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NEET 2025 - Target Batch
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