1. | The cutting of DNA by restriction endonucleases results in fragments of DNA and these fragments can be separated by this technique. |
2. | The most commonly used matrix is agarose which is a natural polymer extracted from seaweeds. |
3. | The DNA fragments resolve according to their size. Hence, the larger the fragment size, the farther it moves. |
4. | The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide. |
1. | Bioprocess engineering | – | Maintenance of sterile ambience |
2. | Genetic engineering | – | Alteration of genetic material |
3. | Downstream processing | – | Biosynthetic stage |
4. | Biosynthetic stage | – | Culturing of recombinant host cells |
Column I | Column II | ||
a. | Salmonella typhimurium | (i) | Source of EcoRI |
b. | Bacillus thuringiensis | (ii) | Used in developing transgenic tobacco plant |
c. | Agrobacterium tumefaciens |
(iii) | Used in creating first recombinant DNA |
d. | Escherichia coli | (iv) | Gene used in creating biopesticide |