EcoRI cuts the DNA between bases _______ and ______ only when the recognition sequence GAATTC is present in the DNA.
Select the correct option to fill the blanks respectively.
1. A and T
2. T and C
3. G and A
4. A and C

Subtopic:  Tools: Enzymes: I | Restriction Enzymes - Main Enzymes |
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How many cycles of PCR are required for obtaining 256 molecules of dsDNA, if your starting material was one molecule of dsDNA?
1. 4
2. 9
3. 128
4. 8
Subtopic:  Polymerase Chain Reaction: PCR |
 58%
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Select the incorrect match with respect to rDNA technology:
1. Bioprocess engineering   –   Maintenance of sterile ambience
2. Genetic engineering   –   Alteration of genetic material
3. Downstream processing   –   Biosynthetic stage
4. Biosynthetic stage   –   Culturing of recombinant host cells
Subtopic:  Bioreactors/Downstream Processing |
 76%
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Match column I with column II and choose the correct option:
Column I Column II
a. Salmonella typhimurium (i) Source of EcoRI
b. Bacillus thuringiensis (ii) Used in developing transgenic tobacco plant
c. Agrobacterium
tumefaciens
(iii) Used in creating first
recombinant DNA
d. Escherichia coli (iv) Gene used in creating biopesticide

1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic:  Restriction Enzymes - Main Enzymes | Transforming Plant & Animal Cell | Restriction Enzymes: Historical Background |
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During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic:  General Design of an rDNA experiment |
 87%
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NEET - 2013
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Assertion (A): Inserting alien DNA in pBR322 plasmid at Pst I makes the transformants sensitive to ampicillin antibiotic.
Reason (R): Restrictions site for Pst I is present on ampR .
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
 
Subtopic:  Tools: Vectors: I | Tools |
 83%
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Assertion (A): DNA ligase plays important role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic:  General Design of an rDNA experiment |
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Assertion (A): Restriction enzymes belong to a larger class of enzymes called nucleases.
Reason (R): Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the DNA
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic:  Restriction Enzymes - Main Enzymes |
 69%
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Assertion (A): An alien DNA is linked with the origin of replication for making multiple identical copies.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic:  General Design of an rDNA experiment |
 80%
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If you want to isolate DNA from a fungal cell, the enzyme that should not be used for the purpose is:
1. Protease
2. RNase
3. DNase
4. Chitinase
Subtopic:  Process of Biotech |
 57%
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