DNA or RNA segment tagged with a radioactive molecule is called

1. vector

2. probe

3. clone

4. plasmid

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

1. Trichoderma harzianum

2. Meloidogyne incognita

3. Agrobacterium tumefaciens

4. Penicillium expansum

Polyethylene glycol method is used for

1. gene transfer without a vector

2. biodiesel production

3. seedless fruit production

4. energy production from sewage

Gel electrophoresis is used for

1. cutting of DNA into fragments

2. separation of DNA fragments according to their size

3. construction of recombinant DNA by joining with cloning vectors

4. isolation of DNA molecule

The linking of antibiotic resistance gene with the plasmid vector became possible with

1. DNA ligase               

2. endonucleases

3. DNA polymerase       

4. exonucleases

Microbes found to be very useful in genetic engineering are:

1. Escherichia coli and Agrobacterium tumefaciens

2. Vibrio cholerae and a tailed bacteriophage

3. Diplococcus sp. and Pseudomonas sp.

4. Crown gall bacterium and Caenorhabditis elegans

Which one of the following palindromic base sequences in DNA can be easily cut at about

the middle by some particular restriction enzyme?

1. 5'$-$CGTTCG$-$3'

     3'$-$ATGGTA$-$-5'

2. 5'$-$GATATG$-$3'

     3'$-$CTACTA$-$5'

3. 5'$-$GAATTC$-$3'

     3'$-$CTTAAG$-$5'

4. 5'$-$CACGTA$-$3'

     3'$-$CTCAGT$-$5'

Which one of the following is used as vector for cloning genes into higher organisms?

1. Baculovirus

2. Salmonella typhimurium

3. Rhizopus nigricans

4. Retrovirus

Continuous addition of sugars in ‘fed batch’ fermentation is done to

1. obtain antibiotics

2. purify enzymes

3. degrade sewage

4. produce methane

Agarose extracted from sea weeds finds use in

1. tissue culture

2. PCR

3. gel electrophoresis

4. spectrophotometry