1. | The main DNA replication enzyme is referred to as DNA-dependent DNA polymerase since it uses a DNA template to catalyse the polymerisation of deoxynucleotides. |
2. | Deoxyribonucleoside triphosphates act as substrates and provide energy for polymerisation reactions. |
3. | Since the two strands of DNA cannot be separated in their entire length, the replication occur within a small opening of the DNA helix, referred to as the replication fork. |
4. | The DNA-dependent DNA polymerases catalyse polymerisation only in one direction, that is 3'→5'. |