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Q. No.The most critical requirement for any DNA molecule to be able to act as a cloning vector during rDNA procedures will be:
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
Subtopic: Cloning Vector |
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Given below are four statements pertaining to the separation of DNA fragments using Gel electrophoresis. Identify the correct statements:
Choose the correct answer from the options given below:
1. Only (c) and (d)
2. Only (a) and (c)
3. Only (b) and (c)
4. Only (a) and (b)
| a: | DNA fragments are negatively charged molecules and can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. |
| b: | The separation of DNA fragments is possible because they differ in size. |
| c: | Smaller the size of the DNA fragment, shorter the distance it travels through agarose gel. |
| d: | Pure DNA can be visualized directly by exposing it to UV radiation without any staining. |
1. Only (c) and (d)
2. Only (a) and (c)
3. Only (b) and (c)
4. Only (a) and (b)
Subtopic: Separation and Isolation of DNA fragments |
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic: Separation and Isolation of DNA fragments |
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NEET - 2023
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
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NEET - 2023
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In gene gun method used to introduce alien DNA into host cells, microparticles of ___________ metal are used.
| 1. | Silver | 2. | Copper |
| 3. | Zinc | 4. | Tungsten or gold |
Subtopic: Transforming Plant & Animal Cell |
92%
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NEET - 2023
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
Choose the correct answer from the options given below:
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
From NCERT
NEET - 2023
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Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
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NEET - 2023
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Which of the following statement is incorrect above Agrobacterium tumefaciens ?
| 1. | It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. |
| 2. | 'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells. |
| 3. | It transforms normal plant cells into tumor cells |
| 4. | It delivers 'T-DNA' into plant cell |
Subtopic: Transforming Plant & Animal Cell |
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Match List - I with List - II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
52%
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NEET - 2023
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