What is the first step in the Southern Blot technique?

(1) Denaturation of DNA on the gel for hybridization with specific probe

(2) Production of a group of genetically identical cells

(3) Digestion of DNA by restriction enzyme

(4) Isolation of DNA from a nucleated cell such as the one from the scene of crime

Given below is a representation of a kind of chromosomal mutation. What is the kind of mutation represented?

(1) Deletion

(2) Duplication

(3) Inversion

(4) Reciprocal translocation

Which one of the following pairs is correctly matched with regard to the codon and the amino acid coded by it?

(1) UUA - valine

(2) AAA - lysine

(3) AUG - cysteine

(4) CCC - alanine

During portein synthesis in an organism at one point the process comes to a halt. Select the group of the three codons from the following, from which any one of the three colud bring about this halt.

(1) UUU, UCC, UAU

(2) UUC, UUA, UAC

(3) UAG, UGA, UAA

(4) UUG, UCA, UCG

What is incorrect about the following figure representing DNA replication?

(1) The direction of DNA replication in strand (i).

(2) The direction of DNA replication in strand (ii).

(3) Discontinuous replication of strand (i)

(4) Discontinuous replication of strand (ii).

The binding site of tRNA with mRNA & amino acids respectively are

(1) mRNA with DHU loop and amino acid with CCA end.

(2) mRNA with CCA end and amino acid with anticodon loop.

(3) mRNA with anticodon loop and amino acid with DHU loop.

(4) mRNA with anticodon loop and amino acid with CCA end.

Which of the following is correct regarding genetic code?

(1) UUU is the initiation codon which also codes for phenylalanine.

(2) There are 64 triplet codons and only 20 amino acids.

(3) Three random nitrogen bases specify the placement of one amino acid.

(4) UAA is the nonsense codon which also codes for methionine.

Which of the following set of options is used in translation?

(1) hnRNA, tRNA, rRNA

(2) mRNA, tRNA, rRNA

(3) mRNA, tRNA, hnRNA

(4) hnRNA, rRNA, tRNA

In a 3.2 Kbp long piece of DNA, 820 adenine bases were found. What would be the number of cytosine bases?

(1) 780

(2) 1560

(3) 740

(4) 1480

Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given.

A. Electrophoresis of DNA fragments

B. Hybridisation with DNA probe

C. Digestion of DNA by restriction endonucleases

D. Autoradiography

E. Blotting of NDA fragments to nitrocellulose membrane

(1) C – A – B – E – D

(2) C – A – E – B – D

(3) A – E – C – B – D

(4) A – C – E – D – B