1. | Primers anneal to the DNA template |
2. | Taq polymerase adds nucleotides to the annealed primer |
3. | Two strands of the DNA separate |
4. | Temperature is 92ºC |
1. | contain DNA and RNA |
2. | demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane |
3. | are bacteria |
4. | All of the above |
A. | Denaturation of ds‐DNA to genomic DNA tempelate |
B. | Thermostable DNA‐polymerase to enzyme DNA‐polymerase |
C. | In vitro synthesis of region of DNA of interest |
D. | Synthesis of oligonucleotides by primers |
E. | Nucleotides complementary region of DNA |
1. | Primers anneal to the DNA template |
2. | Taq polymerase adds nucleotides to the annealed primer |
3. | Two strands of the DNA separate |
4. | Temperature in 92° C |