Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering? 

(1) Splitting A and B polypeptide chains

(2) Addition of C-peptide to pro-insulin

(3) Getting insulin assembled into mature form

(4) Removal of C-peptide from active insulin

Which technique would you expect to be completely curative in SCID?

(1) Gene therapy in adult stage

(2) Gene therapy in embryonic stage

(3) Bone marrow transplantation

(4) Enzyme replacement therapy

For effective treatment of a disease: 

(1) Early diagnosis is needed but understanding of its pathophysiology is not needed

(2) Early diagnosis is not needed but understanding of its pathophysiology is needed

(3) Early diagnosis and understanding of its pathophysiology are needed

(4) Neither early diagnosis nor understanding of its pathophysiology is needed

"Rosie" a transgenic cow known to produce a type of milk which has all the following characteristics except: 

(1) Protein content of 2.4 gm/litre

(2) Has human α-lactalbumin

(3) More balance diet than normal milk for babies

(4) None of these

Which of the following is correct?

(1) The procedure for chemical safety testing / toxicity is the same as that used for testing toxicity of drugs

(2) Transgenic animals are more sensitive to the toxic substances than non-transgenic animals

(3)  Golden rice, a genetically engineered rice has high vitamin A (β-carotene) content

(4) All are correct

Introduction of genetically modified food is not desirable because: 

(1) Allergies and toxicity may be caused

(2) Incorporation of antibiotic resistance in human beings

(3) Disturbance in metabolism due to enzyme for antibiotic resistance 

(4) All are correct

The use of bioresources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment is called:

(1) Bioethics

(2) Biopiracy

(3) Bioterror

(4) Bioweapon

Which of the following statement is not true: 

(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli

(3)  The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin

(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning

Go through the statements:

A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinsulin.

B. In hydridoma technology, B-cells are fused with myeloma cells.

C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccine before use in humans.

D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.

E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.

F. Gray biotechnology' is referred to industrial process.

G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA. 

How many statement(s) is wrong?

(1) One

(2) Two

(2) Four

(4) Six

Which of the following is / are product(s) of genetic engineering:

A. Humulin

B. Hepatitis B vaccine prepared by yeast

C. Golden rice

D. Bt cotton

(1) A, B

(2) B, C

(3) A, B, C

(4) A, B, C, D