The enzyme used in the polymerase chain reaction is a :

(1) DNA dependent RNA polymerase

(2) RNA dependent DNA polymerase

(3) DNA dependent DNA polymerase

(4) RNA dependent RNA polymerase

Taq polymerase is used in the polymerase chain reaction because:

1. It replicates DNA faster than other enzymes

2. It is the only enzyme that can replicate DNA invitro

3. It does not require an RNA primer to function

4. It is thermostable 

Plants are more easily manipulated by genetic engineering than are animals because:

1. Plant genes do not contain introns

2. More vectors are available to transfer rDNA into plant cells

3. Totipotency is a feature generally associated with plant cells and not with animal cells

4. Genes can be inserted into animal cells by microinjection.

Identify the incorrectly matched pair :

To protect tobacco plants against Meloidegyne incognita using RNAi, nematode specific genes were introduced in the plant using:

1. Transposons

2. Retrotransposons

3. Virus with RNA genome 

4. Agrobacterium

A permanent cure for ADA deficiency could be:

Polymerase Chain Reaction is not used in :

1. Confirming presence of a pathogen during early infection

2. Identifying the mutated genes in suspected cancer patients

3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures

4. Detection of the presence of HIV in suspected AIDS patient

If gene isolate from marrow cells producing ADA is introduced into cells at early embryonic stage, it could be a permanant cure of ADA deficiency because the early embryonic cells :

1. Are more differentiated than the later stage cells

2. Are constantly dividing and do not get differentiated 

3. Are easy to manipulate than the later stage cells

4. Are virtually immortal 

To test the safety of polio vaccine, the organisms used are transgenic :

1. Mice

2. Pigs

3. Monkey 

4. Cattle