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Q. No.Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
Column-I
Column-II
(a)
Heat denaturation
(i)
70-75°C
(b)
Annealing
(ii)
90-98°C
(c)
Primer extension
(iii)
40-60°C
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
66%
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Identify the process in Column-I with the term in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | Amplifications of gene of interest | (i) | Downstream processing |
| (b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
| (c) | Separation and purification of desired product | (iii) | Transformation |
| (d) | Insertion of rDNA into host | (iv) | Micro propagation |
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Bioreactors/Downstream Processing |
71%
From NCERT
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Match the restriction enzyme given in Column-I with their source given in column II and choose the correct option
1. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
| Column-I | Column-II | ||
| (a) | Alu I | (i) | Bacillus amyloliquefaciens H |
| (b) | Bam HI | (ii) | H. influenza Rd. |
| (c) | Eco RI | (iii) | Arthobacter luteus |
| (d) | Hind RI | (iv) | Escherichia coli Ry 13 |
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
Subtopic: Tools: Vectors: I |
83%
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Match the steps of PCR in Column-I with the process in column II and select the correct option
1. (a)-(iii), (b)-(ii), (c)-(i)
2. (a)-(ii), (b)-(iii), (c)-(i)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(i), (c)-(iii)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | addition of nucleotides on template |
| (b) | Annealing | (ii) | separation of two strands of DNA |
| (c) | Primer Extension | (iii) | paring of primer to complementary sequence in DNA |
2. (a)-(ii), (b)-(iii), (c)-(i)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(i), (c)-(iii)
Subtopic: Polymerase Chain Reaction: PCR |
63%
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Match the technique in Column-I with the terms given in column II
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophoresis | (i) | Chrimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Process of Biotech |
80%
From NCERT
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Consider the given two statements:
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: Tools |
76%
From NCERT
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Introduction of an alien DNA into plant host cell is achieved by making them
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
Subtopic: Transforming Plant & Animal Cell |
61%
From NCERT
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Subtopic: Process of Biotech |
91%
From NCERT
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Which of the following does not hold true for restriction enzymes?
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
Subtopic: Restriction Enzymes - Main Enzymes |
80%
From NCERT
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Name the enzymes 'P' and 'Q' that are involved in the processes given below.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
Subtopic: Restriction Enzymes - Main Enzymes |
77%
From NCERT
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