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Q. No.In agarose gel electrophoresis the separated DNA fragments can be visualized only after staining the DNA with a compound known as:
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
Subtopic: Separation and Isolation of DNA fragments |
97%
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic: Process of Biotech |
80%
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
80%
From NCERT
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Consider the given two statements:
| Assertion (A): | The most commonly used bioreactors for biosynthesis are of stirring type. |
| Reason (R): | A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Bioreactors/Downstream Processing |
56%
From NCERT
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
91%
From NCERT
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Introduction & History |
82%
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| Assertion (A): | During the heat shock method, the cells are incubated with recombinant DNA at \(42^{\circ} \mathrm{C}\), following by placing them briefly on ice and then putting if back at \(42^{\circ} \mathrm{C}\). |
| Reason (R): | After heat shock, uptake of DNA through transient pores in the bacterial cell wall takes place. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Transforming Plant & Animal Cell |
From NCERT
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Name the enzymes 'P' and 'Q' that are involved in the processes given below.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
Subtopic: Restriction Enzymes - Main Enzymes |
77%
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Which of the following does not hold true for restriction enzymes?
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
Subtopic: Restriction Enzymes - Main Enzymes |
80%
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Subtopic: Process of Biotech |
91%
From NCERT
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