Q. No.Which of the following would you choose as the safest and least cumbersome selectable marker-
(1) Ampicillin resistance gene
(2) Tetracyclin resistance gene
(3) Kanamycin resistance gene
(4) b Galactosidase gene
(1) Ampicillin resistance gene
(2) Tetracyclin resistance gene
(3) Kanamycin resistance gene
(4) b Galactosidase gene
Recombinant DNA is formed in nature during-
(1) Crossing over at pachytene
(2) Integration of plasmid to form episome
(3) Integration of phage DNA to form prophage
(4) More than one is correct.
Southern Blotting essentially makes use of a
(1) Buffer
(2) Electrodes
(3) Agarose gel
(4) All of the above
The native charge on DNA molecules is
(1) Positive
(2) Negative
(3) Neutral
(4) DNA is an ampholyte
The visualization of the DNA bands on the gel can be done with the help of
(1) UV rays
(2) Ethidium bromide
(3) Chromogenic substrate
(4) More than one is correct
If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?
(1) Any cell of the human body as the DNA is same in all cells
(2) Any nucleated cell of the human body
(3) b cells of islets of Langerhans of human pancreas
(4) WBCs
The natural genetic engineer of plants is
(1) Armillaria ostoyae
(2) Agrobacterium tumefacians
(3) Argemonaria geneticis
(4) Nuclearia incorporate
A distinct advantage of continuous culture over batch culture is
(1) Higher productivity
(2) Versitality
(3) Can be used for different reactions everyday
(4) Continuous inflow of nutrients
Plasmids are good vectors for genetic engineering because
1. They self replicate within bacterial cells
2. Replicate freely outside bacterial cells
3. Can be replicated in culture
4. Can be replicated in laboratory using enzymes
Molecular weight of DNA in diploid human cell in Da is
- 3.6 X 10 12 Da
- 46 X 10 10
- 30 billion Da
- 46 billion Da
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