Which of the following is not associated with first recombinant DNA molecule?

1.      Plasmid of Salmonella typhimurium

2.      Antibiotic resistance gene

3.      1975

4.     Stanley Cohen

If you want to isolate a particular gene from a plasmid or genome of an organism which of the following enzyme has to be used?

1.      DNA ligase

2.      Lysozyme

3.      Restriction enzyme

4.     DNA polymerase

Cohen and Boyer are known for which of the following discoveries?

1.      Antibiotic resistance gene

2.      Recombinant DNA

3.      Restriction Digestion

4.     Plasmid

Arrange the following enzymes/ processes in the order of their use for a certain experiment of genetic engineering:

1) ligase,
2) restriction enzyme
3)desired gene
4) Transformation
5) Expression

1.      3-2-1-4-5

2.      1-2-4-5-3

3.      3-1-4-5-2

4.     2-5-4-3-1

What does cloning mean?

1.      The ability to multiply the desired gene in the host

2.      The ability to multiply the vector in the host

3.      The ability to multiply the antibiotic resistance gene in the host

4.     The ability to multiple Ori in the host

Which of the following is not a tool of RDT?

1.      PCR

2.      Restriction enzymes

3.      Vectors

4.     Host organisms

Which of the following is a tool of RDT?

1.      PCR

2.      Gel Electrophoresis

3.      Cloning

4.     Restriction enzyme

In the year 1963, the two enzymes responsible for inhibiting the growth of bacteriophage in E. coli were isolated. One of them was

1.      Ligase

2.      Polymerase

3.      Molecular scissor

4.     Antibiotic resistance enzyme

Hind II

1.      First restriction endonuclease

2.      Was characterized 7 years later

3.      Specific sequence of 5 base pairs

4.     All of these

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1.      700; 350

2.      900; 230

3.      600; 270

4.     550; 210