What is true about the steps of PCR shown in the diagram given below?
  

I:	Step A occurs at the temperature of 95 – 980 C
II:	Step B occurs at the temperature of 550 C
III:	Step C occurs at the temperature of 720 C

 

1.	I and II only	2.	I and III only
3.	II and III only	4.	I, II and III

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as:

1. southern blotting
2. northern blotting
3. polymerase chain reaction
4. DNA replication

Agrobacterium tumefaciens is

1. a disease in humans that causes loss of sight
2. a bacterium that can be used to introduce DNA into plants
3. a fungi that is used to produce antibiotics in large amounts
4. a disease in humans that causes loss of weight

Vectors are

1. molecules that degrade nucleic acids
2. molecules that help in replication
3. molecules that are able to covalently bond to and carry foreign DNA into cells
4. molecules that protect host cells from invasion by foreign DNA

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

1. DNA polymerase
2. Restriction endonuclease
3. DNA ligase
4. DNA helicase

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

1. laser

2. DNA probe

3. gene gun

4. inoculating needle

Bacterial resistance to antibiotics is a genetic trait carried in the bacterial:

1. Intron

2. Chromosome

3. Plasmid

4. Centromere

An important limitation to the use of Agrobacterium tumefaciens is that it can not:

1. infect dicots

2. be genetically modified

3. be cultured on a nutrient medium

4. infect crop plants such as wheat and corn

If a sample has a DNA of size 2kb and 6kb while the molecular marker denotes 4kb band as its the first band where will be the 6kb band of DNA?

1. bove the band of another DNA sample

2. Above the band of marker

3. In between the band of the marker and another band of DNA sample.

4. Both A and B

Which of the following is the reason for the increased surface area for oxygen transfer in a sparged bioreactor ?

1. Bubbles

2. Turbidity

3. Cells

4. Protein