Q. No.A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1.
Eco Rl
2.
taq polymerase
3.
polymerase lll
4.
ligase
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
| 1. | Vector | 2. | Plasmid |
| 3. | Structural gene | 4. | Selectable marker |
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
2. DNase I
3. RNase
4. Hind II
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
1. Plant cells- cellulose
2. Algae - Methylase
3. Fungi - Chitinase
4. Bacteria - Lysozyme
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
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