A gene whose expression helps to identify transformed cell is known as:
1. | Vector | 2. | Plasmid |
3. | Structural gene | 4. | Selectable marker |
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
1. | The smaller the fragment size, the farther it moves |
2. | Positively charged fragments move to farther end |
3. | Negatively charged fragments do not move |
4. | The larger the fragment size, the farther it moves |
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
1. Plant cells- cellulose
2. Algae - Methylase
3. Fungi - Chitinase
4. Bacteria - Lysozyme
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
The colonies of recombinant bacteria appear white because of:
1. | Insertional inactivation of alpha-galactosidase in non-recombinant bacteria |
2. | Insertional inactivation of beta-galactosidase in recombinant bacteria |
3. | Inactivation of glycosidase enzyme in recombinant bacteria |
4. | Non-recombinant bacteria containing beta-galactosidase |
The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes