The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperatures

Restriction endonucleases are enzymes which:

In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with

1. corn

2. rice

3. wheat

4. soy beans

In the screening process, clones that metabolize X-gal turn

1. yellow

2. orange

3. blue

4. colorless

Restriction endonuclease:

1. cuts the DNA molecule randomly

2. cuts the DNA molecule at specific sites

3. restricts the synthesis of DNA inside the nucleus

4. synthesis DNA

The vector for T-DNA is:

1. Thermus aquaticus

2. Salmonella typhimurium

3. Agrobacterium tumefaciens

4. Escherichia coli

In recombinant DNA technique, the term vector refers to:

1. Donor DNA, is identified and picked up through electrophoresis

2. Plasmid transfers DNA into living cell

3. Collection of the entire genome in the form of plasmid

4. Enzymes cut the DNA at specific sites