Q. No.Gel electrophoresis is used for:
1.
cutting of DNA into fragments
2.
separation of DNA fragments according to their size
3.
construction of recombinant DNA by joining with cloning vectors
4.
isolation of DNA molecule
| 1. | cutting of DNA into fragments |
| 2. | separation of DNA fragments according to their size |
| 3. | construction of recombinant DNA by joining with cloning vectors |
| 4. | isolation of DNA molecule |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
Restriction endonuclease:
1. cuts the DNA molecule randomly
2. cuts the DNA molecule at specific sites
3. restricts the synthesis of DNA inside the nucleus
4. synthesis DNA
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol
3. Chilled ethanol
4. Methanol at room temperature
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
Stirred-tank bioreactors have been designed for:
1. Addition of preservatives to the product
2. Purification of the product
3. Ensuring anaerobic conditions in the culture vessel
4. Availability of oxygen throughout the process
Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
| 1. | 5'-CGTTCG-3' | 2. | 5'-GATATG-3' |
| 3'-ATGGTA-5' | 3'-CTACTA-5' | ||
| 3. | 5'-GAATTC-3' | 4. | 5'-CACGTA-3' |
| 3'-CTTAAG-5' | 3'-CTCAGT-5' |
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
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