Restriction endonuclease:

1. cuts the DNA molecule randomly

2. cuts the DNA molecule at specific sites

3. restricts the synthesis of DNA inside the nucleus

4. synthesis DNA

The vector for T-DNA is:

1. Thermus aquaticus

2. Salmonella typhimurium

3. Agrobacterium tumefaciens

4. Escherichia coli

In recombinant DNA technique, the term vector refers to:

1. Donor DNA, is identified and picked up through electrophoresis

2. Plasmid transfers DNA into living cell

3. Collection of the entire genome in the form of plasmid

4. Enzymes cut the DNA at specific sites

Given below is a sample of a portion of the DNA strand given the base sequence on the opposite strand. What is so special show in it? 
5'- GAATTC...3' 
3'- CTTAAG ... 5'

1. Detection mutation

2. Start codon at 5' end

3. Palindromic sequence of base pairs

4. Replication completed

A kind of biotechnology involving manipulation of DNA is:

1. DNA replication

2. genetic engineering

3. denaturation

4. renaturation

The thermostable enzymes, Taq and Pfu, Isolated from thermophilic bacteria are:

1. RNA polymerases

2. DNA ligases

3. DNA polymerases

4. Restriction endonucleases

Biolistic technique is used in:

1. Gene transfer process

2. Tissue culture process

3. Hybridization process

4. Germplasm conservation process

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:

DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol 
3. Chilled ethanol
4. Methanol at room temperature

Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?